how can i shorten this program??

[CCGG26]

map = {}
dna = [ "A", "C", "G", "T"]

with open("matrix.txt", "r") as myfile:
    line = myfile.readline()
    mylist = line.split()
    map{"AA"} = mylist[0]
    map{"AC"} = mylist[1]
    map{"AG"} = mylist[2]
    map{"AT"} = mylist[3]
    line = myfile.readline()
    mylist = line.split()
    map{"CA"} = mylist[0]
    map{"CC"} = mylist[1]
    map{"CG"} = mylist[2]
    map{"CT"} = mylist[3]
    line = myfile.readline()
    mylist = line.split()
    map{"GA"} = mylist[0]
    map{"GC"} = mylist[1]
    map{"GG"} = mylist[2]
    map{"GT"} = mylist[3]
    line = myfile.readline()
    mylist = line.split()
    map{"TA"} = mylist[0]
    map{"TC"} = mylist[1]
    map{"TG"} = mylist[2]
    map{"TT"} = mylist[3]
gap = -2
map{"A-"} = gap
map{"C-"} = gap
map{"T-"} = gap
map{"G-"} = gap
map{"-A"} = gap
map{"-C"} = gap
map{"-T"} = gap
map{"-G"} = gap
with open("dna.fasta.txt","r") as myfile:
    data = myfile.readlines()
    myfile.close()

 
for i in range(len(data)):
    data[i] = data[i].rstrip("\n")
         
seq1 = data[1]
seq2 = data[3]
score = 0

      
for i in range(0,len(seq1),1):
         
        key = seq1[i] + seq2[i]
        score = score + int(map[key])
      
print(score)

[bvdet]

Here's a way to shorten some of it.

myfile = '''0 1 2 3
4 5 6 7
8 9 10 11
12 13 14 15'''

myfileList = myfile.split("\n")
dna = [ "A", "C", "G", "T"]

dnaDict = {}
for j, a in enumerate(dna):
    itemList = myfileList[j].split()
    for i, b in enumerate(dna):
        dnaDict["%s%s" % (a, b)] = itemList[i]

The example approximates reading the data from a file.